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KMID : 0903519890320030270
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1989 Volume.32 No. 3 p.270 ~ p.277
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Mechanism of Action of Prochloraz and Triadimefon on Valsa ceratosperma
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Abstract
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In order to elucidate the action mechanism of prochloraz and triadimefon, the mycelia of the Valsa ceratosperma were treated with the compounds in vitro.
Analysis by GLC of the sterol extract from Valsa ceratosperyna mycelia revealed one major peak and two minor peaks. Their relative retention time(RRT) relative to chloresterol were 1.29, 1.48 and 1.82, The compounds with RRT 1.29 and RRT 1.82 were identified as ergosterol and 24-methylenedihydrolanosterol by GC/MS, respectively.
Five ppm of prochloraz and triadimefon applied to mycelia caused decrease in ergosterol content, whereas increase in 24-methylenedihydrolanoserol content in mycelia.
The longer treatment time and the higher concentrations of the chemicals resulted in the greater decrease in ergosterol and the greater increase in 24-methylenedihydrolanosterol. Based on the analysis, it is considered that the two chemicals inhibit the ergosterol biosynthesis in Valsa ceratosperma by blocking C-14 demethylation as found previously in other fungi arid yeasts.
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